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Anti-TRIB3 Monoclonal Antibody
Catalog Number: orb547738
| Catalog Number | orb547738 |
|---|---|
| Category | Antibodies |
| Description | Anti-TRIB3 Monoclonal Antibody. Tested in WB, IP applications. This antibody reacts with Human. |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | AEIE-20 |
| Tested applications | IP, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding ly to Akt kinases and blocking their activation. May bind ly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 40 kDa |
| UniProt ID | Q96RU7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | RalA-binding protein 1; RalBP1; 76 kDa Ral-interac Read more... |
| Note | For research use only |
| Application notes | WB 1:1000-1:5000IP 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of TRIB3 expression in 293T cell lysate using anti-TRIB3 antibody (orb547738). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB3 antigen affinity purified polyclonal antibody (Catalog # orb547738) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for TRIB3.
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