TREM1/CD354 Antibody

• Catalog Number: orb1805967
• Category: Antibodies
• Description: Anti-TREM1 / CD354 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Target: TREM1 / CD354
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/mL
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Purity: > 95%
• Purification: Protein A
• UniProt ID: Q9NP99
• MW: 143.66 kDa
• Tested applications: ELISA, FA, FACS, In vivo
• Expression System: CHO
• Endotoxins: < 0.001 EU/ug
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Anti-TREM1 / CD354 Reference Antibody (PY159) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TREM1 / CD354 Reference Antibody (PY159)is more than 95% ;determined by SEC-HPLC.

Immobilized human TREM1 His at 2 μg/mL can bind Anti-TREM1 / CD354 Reference Antibody (PY159);EC50=0.00645μg/mL.

Human TREM1 HEK293 cells were stained with Anti-TREM1 / CD354 Reference Antibody (PY159) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC275=0.7744nM.

The endocytosis ratio PY159 by hu-TREM1-HEK293 increased with the increase of antibody concentration; and the Internalization Rate (%) reached 80% at antibody concentration of 0.3 nM.

Anti-TREM1 Reference Antibody (PY159) Activation was evaluated using PBMC. The max induction fold was approximately 3.16.