Anti-Transferrin receptor [R17 217.1.3]

• Catalog Number: orb758840
• Category: Antibodies
• Description: Rat monoclonal antibody to CD71
• Target: Transferrin receptor
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
• UniProt ID: Q62351
• Tested applications: FC
• Clone Number: R17 217.1.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD71; Transferrin receptor protein 1; TR; ; TfR1;
• Note: For research use only

Flow-cytometry using the Anti-Transferrin receptor antibody R17 217.1.3. RAW 264.7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of R17 217.1.3 (orb758839, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody R17 217.1.3. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of R17 217.1.3 (orb758839) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758839, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.