Anti-TOM20 Antibody

• Catalog Number: orb214933
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TOMM20
• Target: TOMM20
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TOM20. The exact sequence is proprietary.
• UniProt ID: Q15388, Q62760, Q9DCC8
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti KIAA0016 antibody, anti Mitochondrial import
• Note: For research use only
• Entrez: 266601, 9804, 67952

Western blot analysis of TOM20 expression in HEK293T (A), Hela (B), H460 (C), mouse lung (D), mouse spleen (E), mouse kidney (F), rat spleen (G) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 20 kD)

Immunofluorescent analysis of TOM20 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).