Anti-TNFSF7 / CD27L / CD70 Reference Antibody (vorsetuzumab)

• Catalog Number: orb1806368
• Category: Antibodies
• Description: Anti-TNFSF7 / CD27L / CD70 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 150 kDa
• Target: TNFSF7 / CD27L / CD70
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-TNFSF7 / CD27L / CD70 Reference Antibody (vorsetuzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TNFSF7 / CD27L / CD70 Reference Antibody (vorsetuzumab)is more than 99.29% ;determined by SEC-HPLC.

HL-60 cells were stained with Anti-TNFSF7 / CD27L / CD70 Reference Antibody (vorsetuzumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC150=0.06731 ug/mL.

The endocytosis ratio vorsetuzumab by 786-O increased with the increase of antibody concentration; and the Internalization Rate (%) reached 55% at antibody concentration of 0.67 nM.