TNFRSF5/CD40 Antibody

SKU: orb1806256

Description

Anti-TNFRSF5 / CD40 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Target: TNFRSF5 / CD40
• Source/Expression System: CHO
• Molecular Weight: 150 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab)is more than 99.21% ;determined by SEC-HPLC.

Immobilized human TNFRSF5 / CD40 His at 2 μg/mL can bind Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab);EC50=0.0252 μg/mL.

Human CD40 CHO cells were stained with Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC77=0.6311 ug/mL.

Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab);FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 1.359 ug/mL.

Quick Database Links

Gene Symbol

TNFRSF5 / CD40

UniProt

P25942