Anti-Tim-3 [2C12]

• Catalog Number: orb758816
• Category: Antibodies
• Description: Rat monoclonal antibody to Tim-3
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 2C12
• Tested applications: Blocking, FC, IF, IHC
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund’s adjuvant.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Tim-3
• UniProt ID: Q8VIM0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD366; HAVcr-2; HAVCR2; Hepatitis A virus cellular
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-TIM-3 antibody on TIM-3-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-3 antibody 2C12 (orb758815; blue line) and isotype control (orb256390, anti-Fluorescein; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using orb758815. For signal detection, anti-rabbit IgG1 HRP antibody was used.

Flow cytometry using the Anti-Tim-3 antibody 2C12. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 2C12 (orb758815, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 2C12 (orb758815) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758815, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.