Anti-Tim-3 [2C12]

SKU: orb758816

Description

Rat monoclonal antibody to Tim-3

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, FC, IF, IHC
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 2C12
• Immunogen: This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund’s adjuvant.
• Target: Tim-3
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD366; HAVcr-2; HAVCR2; Hepatitis A virus cellular receptor 2 homolog; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3; TIM3; 8B.2C12

ELISA of anti-TIM-3 antibody on TIM-3-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-3 antibody 2C12 (orb758815; blue line) and isotype control (orb256390, anti-Fluorescein; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using orb758815. For signal detection, anti-rabbit IgG1 HRP antibody was used.

Flow cytometry using the Anti-Tim-3 antibody 2C12. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 2C12 (orb758815, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 2C12 (orb758815) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758815, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Tim-3

UniProt

Q8VIM0