Anti-TIM-2 [RMT2-14]

SKU: orb758823

Description

Rabbit monoclonal antibody to TIM-2

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: Blocking, FA, FC, IHC, WB
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG
• Clone No.: RMT2-14
• Immunogen: This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
• Target: TIM-2
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIMD2; T Cell Immunoglobulin Mucin-2; T Cell immunoglobulin and mucin domain protein 2; TIM3

Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RMT2-14 (orb758823) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758823, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

TIM-2

UniProt

Q8R183