Anti-Tim-1 [3B3]

• Catalog Number: orb613962
• Category: Antibodies
• Description: Rabbit monoclonal antibody to KIM1
• Target: Tim-1
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1–Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1–Ig in PBS.
• UniProt ID: Q5QNS5
• Tested applications: FC, IF, IHC
• Clone Number: 3B3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD365; HAVcr-1; HAVCR1; Hepatitis A virus cellular
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (orb613962; red line) and isotype control (orb256390, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using orb613962. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.

Surface plasmon resonance (SPR) sensorgram anti-TIM-1 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 (orb613962).