Anti-TIGIT Reference Antibody (tiragolumab)

• Catalog Number: orb1806303
• Category: Antibodies
• Description: Anti-TIGIT Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 150 kDa
• Target: TIGIT
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-TIGIT Reference Antibody (tiragolumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TIGIT Reference Antibody (tiragolumab)is more than 95.79% ;determined by SEC-HPLC.

Immobilized human TIGIT His at 2 μg/mL can bind Anti-TIGIT Reference Antibody (tiragolumab);EC50=0.01643μg/mL.

Human TIGIT HEK293 cells were stained with Anti-TIGIT Reference Antibody (tiragolumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC111=0.2053 μg/mL.

Anti-TIGIT Reference Antibody (tiragolumab)-induced TIGIT Luciferase activity was evaluated using hu-TIGIT-CD226-NFAT-Jurkat;Reporter Cell. The EC50 was approximately 4.978nM.