Thymine DNA Glycosylase Rabbit Polyclonal Antibody

SKU: orb224021

Description

The Thymine DNA Glycosylase Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Thymine DNA Glycosylase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Thymine DNA Glycosylase. The exact sequence is proprietary.
• Target: TDG
• Molecular Weight: 46 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; hTDG

Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of paraffin-embedded mouse testis using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse thymus using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:100.

Western blot analysis of mouse brain (1) using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:1000.

Quick Database Links

Gene Symbol

TDG

UniProt

Q13569, P56581

NCBI Gene ID

6996, 21665