Anti-TGFalpha [tAb2]

• Catalog Number: orb613873
• Category: Antibodies
• Description: Mouse monoclonal antibody to TGFalpha
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: tAb2
• Tested applications: DOT, ELISA
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Biologically active hemocyanin-coupled human TGFalpha.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TGFalpha
• UniProt ID: P01135
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TGFA, Transforming growth factor A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-TGF-alpha antibody tAb2 Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (orb613874, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.