Anti-Tetraspanin 1 [4/12]

• Catalog Number: orb758895
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Tetraspanin 1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4/12
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising BALB/c mice with the major extracellular domain of tetraspanin 1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Tetraspanin 1
• UniProt ID: O60635
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Net1; C4.8; Tetraspanin-1; Tspan-1; TSPAN1; Tetras
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-Tetraspanin 1 antibody 4/12. Paraformaldehyde fixed A431 cells permeabilized with 0.5% triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 4/12 (orb758895, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed A431 cells with anti-Tetraspanin 1 antibody 4/12. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version 4/12 (orb758895) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758895, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.