Anti-TCR [H57-597]

• Catalog Number: orb1152496
• Category: Antibodies
• Description: Hamster monoclonal antibody to TCR
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: H57-597
• Tested applications: FC, IHC, IP
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TCR
• UniProt ID: P01852
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TCR-beta; T cell receptor; T-cell receptor; T cell
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-TCR antibody H57-597. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of H57-597 (orb1152495, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-TCR H57-597. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of H57-597 (orb1152495) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152495, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.