Anti-TCR betaF1 [38C2]

SKU: orb1906347

Description

Anti-TCR betaF1 [38C2]

Research Area

Cancer Biology, Molecular Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 38C2
• Immunogen: The original antibody was generated by immunizing mice with TCRβF1 protein.
• Target: TCR betaF1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCRβF1; T cell antigen receptor markers βf1; CGMCCNO 20778

Immunofluorescence staining of Jurkat cells with anti-TCR betaF1 antibody 38C2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 38C2 (orb1906346) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906346, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-TCR betaF1 antibody 38C2. Jurkat (A) and Molt4 (B) cells lysates (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 38C2 (orb1906346) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Quick Database Links

Gene Symbol

TCR betaF1