Anti-TAU (Phospho-S673) Antibody

• Catalog Number: orb256674
• Category: Antibodies
• Description: Rat polyclonal antibody to Tau having MW of 82 kD that helps stabilize microtubules in our body. On splicing MAPT one can obtain tau protein. Majority of them are found in the CNS region within the neurons but they are not abundant in astrocytes and oligodendrocytes. Tau proteins exist as six different isoforms in brain tissue. These can be distinguished from each other based on their binding domains. If tau protein is no longer functioning we may develop disorders that affect the nervous system such as Alzheimer's and Parkinson's disease. These proteins are very rarely found in non-neuronal cells and are mainly active in the axon part of the neuron.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S673 of human TAU protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPT
• Entrez: 4137, 17762
• UniProt ID: P10637, P10636, P19332
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-AI413597 antibody, anti- AW045860 antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TAU (Phospho-S673) expression in mouse kidney (A), mouse muscle (B), rat muscle (C) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 50-80 kD)

Immunohistochemical analysis of TAU (Phospho-S673) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TAU (Phospho-S673) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).