SYP/Synaptophysin Mouse Monoclonal Antibody

SKU: orb623778

Description

Anti-SYP/Synaptophysin Antibody (monoclonal, 3G12). Tested in WB applications. This antibody reacts with Mouse, Rat.

Research Area

Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Mouse, Rat
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 3G12
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.
• Target: Mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51/52
• Molecular Weight: 38 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MRXSYP; Major synaptic vesicle protein p38

Western blot analysis of SYP using anti-SYP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain whole cell lysates, Lane 3: mouse brain whole cell lysates, Lane 4: mouse brain whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody at 0.5 g/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SYP at approximately 38 KD. The expected band size for SYP is at 34 KD.

Quick Database Links

Gene Symbol

Mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51/52

UniProt

P07825, P08247, Q62277