Anti-SYNE3 Antibody

• Catalog Number: orb215388
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SYNE3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SYNE3. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SYNE3
• Entrez: 212073, 161176
• UniProt ID: Q6ZMZ3, Q4FZC9
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti C14orf49 antibody, anti Nesprin-3 antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SYNE3 expression in AML12 (A), H9C2 (B), A549 (C), HepG2 (D), HEK293T (E) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 112 kD)

Immunofluorescent analysis of SYNE3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.