Anti-Synaptophysin Antibody

• Catalog Number: orb1474244
• Category: Antibodies
• Description: Mouse monoclonal antibody to Synaptophysin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Purified recombinant fragment of SYP expressed in E. Coli.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: SYP
• Entrez: 20977, 6855, 24804
• UniProt ID: P07825, Q62277, P08247
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: Synaptophysin; Major synaptic vesicle protein p38
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)

Immunohistochemical analysis of Synaptophysin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.140). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.