Anti-Swiprosin-2 Antibody

SKU: orb323197

Description

Mouse monoclonal antibody to Swiprosin-2

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:1000-1:2000, IF/ICC: 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Swiprosin-2. The exact sequence is proprietary.
• Target: EFHD1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SWS2 antibody, anti EF-hand domain-containing protein D1 antibody, anti EF-hand domain-containing protein 1 antibody, anti Swiprosin-2 antibody

Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)

Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

EFHD1

UniProt

Q9D4J1, Q9BUP0

NCBI Gene ID

80303, 98363