Anti-SUV420H2 [RAB-C409]

• Catalog Number: orb1089922
• Category: Antibodies
• Description: Human polyclonal antibody to SUV420H2
• Target: SUV420H2
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Fab fragment
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing SUV420H2 protein under non-denaturing conditions.
• UniProt ID: Q86Y97
• Tested applications: ChIP, ELISA
• Clone Number: RAB-C409
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Suv4-20h2; Suppressor of variegation 4-20 homolog
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only

Flow cytometry using the anti-SUV420H2 antibody RAB-C409. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RAB-C409 (orb1089945, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-C409 (orb1089945) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom orb1089945, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.