Sterol carrier protein 2 Rabbit Monoclonal Antibody

SKU: orb866849

Description

Anti-Sterol carrier protein 2 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Disease Biomarkers, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 28S05
• Immunogen: A synthesized peptide derived from human Sterol carrier protein 2
• Target: Sterol carrier protein 2
• Molecular Weight: 59 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP synthase subunit beta, mitochondrial; 3.6.3.14; ATP5B; ATPMB, ATPSB

Western blot analysis of Sterol carrier protein 2 using anti-Sterol carrier protein 2 antibody (orb866849). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Sterol carrier protein 2 antigen affinity purified monoclonal antibody (Catalog # orb866849) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for Sterol carrier protein 2 at approximately 58 kDa. The expected band size for Sterol carrier protein 2 is at 58 kDa.

Quick Database Links

Gene Symbol

Sterol carrier protein 2

UniProt

P22307