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Anti-STAT5A (Phospho-S780) Antibody

Catalog Number: orb214623

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb214623
CategoryAntibodies
DescriptionRabbit polyclonal antibody to STAT5A
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, IP, WB
ReactivityHuman, Primate
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S780 of human STAT5A protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSTAT5A
Entrez6776
UniProt IDP42229
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti STAT5 antibody, anti Signal transducer and ac
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-STAT5A (Phospho-S780) Antibody

Western blot analysis of STAT5A (Phospho-S780) expression in A431 EGF-treated (A), HepG2 (B) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 90 kD)

Anti-STAT5A (Phospho-S780) Antibody

Immunohistochemical analysis of STAT5A (Phospho-S780) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-STAT5A (Phospho-S780) Antibody

Immunofluorescent analysis of STAT5A (Phospho-S780) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.