STAT1 Antibody

SKU: orb44490

Description

Mouse Monoclonal to STAT1.

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: IHC-P, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: lung macrophages.Western blotting: Recommended dilution 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: SM1
• Immunogen: STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
• Target: STAT1
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CANDF7, IMD31, ISGF3

Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot. Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).

Western blotting analysis of human STAT1 using mouse monoclonal antibody SM1 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-STAT1 followed by IRDye800-conjugated anti-mouse secondary antibody. STAT1 was detected at approximately 90 kDa.

Quick Database Links

Gene Symbol

STAT1

UniProt

P42224

NCBI Gene ID

6772