Cart summary

You have no items in your shopping cart.

Anti-STAT1 (Phospho-S727) Antibody

Catalog Number: orb214616

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb214616
CategoryAntibodies
DescriptionRabbit polyclonal antibody to STAT1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, IP, WB
ReactivityHuman, Mouse, Porcine, Primate, Rat, Sheep
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT1 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSTAT1
Entrez6772
UniProt IDP42224, P42225
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Signal transducer and activator of transcript
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-STAT1 (Phospho-S727) Antibody

Western blot analysis of STAT1 (Phospho-S727) expression in A431 (A), NIH3T3 insulin-treated (B), Jurkat pervanadate-treated (C) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 91 kD)

Anti-STAT1 (Phospho-S727) Antibody

Immunohistochemical analysis of STAT1 (Phospho-S727) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-STAT1 (Phospho-S727) Antibody

Immunofluorescent analysis of STAT1 (Phospho-S727) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Stat1 (phospho-S727) polyclonal antibody [orb644768]

    ICC,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 25 μl, 100 μl, 50 μl
  • Stat1 (phospho-S727) polyclonal antibody [orb644791]

    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 25 μl, 100 μl, 50 μl