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Anti-SOX9 Antibody

Catalog Number: orb315709

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb315709
CategoryAntibodies
DescriptionRabbit polyclonal antibody to SOX9
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IH, IP, WB
ReactivityHuman, Mouse, Porcine, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOX9. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500, IP: 1-10:100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSOX9
Entrez20682, 6662
UniProt IDQ04887, P48436
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Transcription factor SOX-9 antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SOX9 Antibody

Western blot analysis of SOX9 expression in MCF7 (A), Panc1 (B), 3T3L1 (C), A375 (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 56 kD)

Anti-SOX9 Antibody

Immunohistochemical analysis of SOX9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SOX9 Antibody

Immunofluorescent analysis of SOX9 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    Polyclonal

    Unconjugated

    100 μg
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    ELISA,  IF,  IHC-P,  WB

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    Polyclonal

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    ELISA,  IHC-P,  WB

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    Polyclonal

    Unconjugated

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    ELISA,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl