Anti-Softag 1 [NT73]

SKU: orb1821646

Description

Anti-Softag 1 [NT73]

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: E. coli

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: NT73
• Target: Softag 1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rpoC; tabB; epitope tag (SLAELLNAGLGGS); DNA-directed RNA polymerase subunit beta, RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta

ELISA using NT73 and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Anti-Softag 1 antibody NT73 (orb1821647; purple line) and isotype control (orb256381, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 at a concentration of 5 ug/ml.

Immunofluorescence staining of HEK293 cells with Anti-Softag 1 NT73. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Softag 1 expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of NT73 (orb1821646) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

Western Blot using Anti-Softag 1 antibody NT73. Softag 1 expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of NT73 (orb1821646) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

Softag 1

UniProt

P0A8T7