SOD2 Antibody [KO/KD Validated]

• Catalog Number: orb1474088
• Category: Antibodies
• Description: The SOD2 Antibody [KO/KD Validated] is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human SOD2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: Recombinant fusion protein of human SOD2. The exact sequence is proprietary.
• UniProt ID: P04179, P09671
• Tested applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Superoxide dismutase [Mn], mitochondrial
• Note: For research use only
• Entrez: 6648, 20656
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SOD2 expression in human brain (A), Hela (B), MCF7 (C), NIH3T3 (D), SHSY5Y (E) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunohistochemical analysis of SOD2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of A549 cells using Anti-SOD2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.