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Anti-SNF2H SMARCA5 Monoclonal Antibody

Catalog Number: orb548086

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Catalog Numberorb548086
CategoryAntibodies
DescriptionAnti-SNF2H SMARCA5 Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberAFDB-19
Tested applicationsICC, IF, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human SNF2H Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails.
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW250 kDa
UniProt IDO60264
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesWAP four-disulfide core domain protein 2; Epididym
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NoteFor research use only
Application notesWB 1:500-1:2000ICC/IF 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-SNF2H SMARCA5 Monoclonal Antibody

Western blot analysis of SMARCA5 using anti-SMARCA5 antibody (orb548086) in MCF7 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA5 antigen affinity purified polyclonal antibody (Catalog # orb548086) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.