Anti-SNCG [SAIC-31A-10]

• Catalog Number: orb1089985
• Category: Antibodies
• Description: Rabbit monoclonal antibody to SNCG
• Target: SNCG
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original antibody was raised against the KLH-conjugated peptide "TVEEAENIAVTSGVVR", derived from Gamma-synuclein, which plays a role in neurofilament network integrity.
• UniProt ID: Q6FHG5
• Tested applications: ELISA, IHC, WB
• Clone Number: SAIC-31A-10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Synuclein gamma variant; Synuclein, gamma (Breast
• Note: For research use only

Flow cytometry using the Anti-SNCG antibody SAIC-31A-10. Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (orb1089985, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U251 cells with anti-SNCG antibody SAIC-31A-10. Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-31A-10 (orb1089985) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089985, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.