SMYD5 Antibody

SKU: orb412435

Description

The SMYD5 Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human SMYD5 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: WB
• Dilution range: WB (1/500 - 1/1000)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human SMYD5
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

RAI15; SET and MYND domain-containing protein 5; Protein NN8-4AG; Retinoic acid-induced protein 15

Western blot analysis of SMYD5 expression in MCF7 (A), Hela (B), mouse skeletal muscle (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 48 kD)

Immunofluorescent analysis of SMYD5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q6GMV2

NCBI Gene ID

10322