Anti-SMIT1 Antibody

• Catalog Number: orb304590
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMIT1 also known as Na(+)/myo-inositol cotransporter and SLC5A3 whose expression can be detected in tumors but is highly expressive in the cerebral white matter of oligodendrocyte progenitor cells. It is found in the multi pass membrane. This protein is involved in the prevention of intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function. It also helps in the regulation of respiratory gaseous exchange and peripheral nervous system development.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Porcine, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMIT1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SLC5A3
• Entrez: 6526, 53881
• UniProt ID: P53794, Q9JKZ2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-Sodium/myo-inositol cotransporter antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMIT1 expression in human prostate (A), rat kidney (B) whole cell lysates. (Predicted band size: 79 kD; Observed band size: 80 kD)

Immunohistochemical analysis of SMIT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.