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Anti-SMAD7 Antibody

Catalog Number: orb1289726

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1289726
CategoryAntibodies
DescriptionRabbit Polyclonal antibody to SMAD7
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SMAD7. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSMAD7
Entrez81516, 4092, 17131
UniProt IDO35253, O88406, O15105
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesMADH7; MADH8; Mothers against decapentaplegic homo
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SMAD7 Antibody

Western blot analysis of SMAD7 expression in mouse brain (A) whole cell lysates. (Predicted band size: 23; 46 kD; Observed band size: 46 kD)

Anti-SMAD7 Antibody

Immunohistochemical analysis of SMAD7 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SMAD7 Antibody

Immunofluorescent analysis of SMAD7 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    ELISA,  IHC-P,  WB

    Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
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    WB

    Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Anti-SMAD7 (Acetyl-K70) Antibody [orb665848]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 30 μl
  • Smad7 antibody (FITC) [orb16325]

    ICC,  IF

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    FITC

    100 μg
  • Anti-SMAD7 (AcK70) Antibody [orb1421714]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl