Anti-SMAD5 Antibody

• Catalog Number: orb1474380
• Category: Antibodies
• Description: Mouse monoclonal antibody to SMAD5
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Human
• Immunogen: Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), FC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: SMAD5
• Entrez: 4090
• UniProt ID: Q99717
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: MADH5; Mothers against decapentaplegic homolog 5;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD5 expression in Hela (A), Jurkat (B), K562 (C) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)

Immunofluorescent analysis of SMAD5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Flow cytometric analysis of SMAD5 in Jurkat cells (red). Black line histogram represents the isotype control.