Smad3 Rabbit Polyclonal Antibody

SKU: orb251579

Description

Anti-Smad3 Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Smad3, identical to the related mouse and rat sequences.
• Target: Mothers against decapentaplegic homolog 3
• Molecular Weight: 50-55 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Anti-SMAD3 Picoband antibody, Western blotting. All lanes: Anti SMAD3 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted bind size: 48 KD. Observed bind size: 56 KD.

Quick Database Links

Gene Symbol

Mothers against decapentaplegic homolog 3

UniProt

P84022