Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human Smad3, identical to the related mouse and rat sequences.
Target	Mothers against decapentaplegic homolog 3
Molecular Weight	48 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

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Anti-SMAD3 Picoband antibody, Western blotting. All lanes: Anti SMAD3 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted bind size: 48 KD. Observed bind size: 56 KD.

Quick Database Links

Gene Symbol

Mothers against decapentaplegic homolog 3

UniProt

P84022

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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