Smad2 Rabbit Polyclonal Antibody

SKU: orb97024

Description

Anti-Smad2 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2, identical to the related rat and mouse sequences.
• Target: Mothers against decapentaplegic homolog 2
• Molecular Weight: 60 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2, MADR2

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: HELA Cell Lysate, Lane 3: SMMC Cell Lysate, Lane 4: JURKAT Cell Lysate, Lane 5: U87 Cell Lysate, Lane 6: MCF-7 Cell Lysate using Smad2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using Smad2 antibody

Confocal immunofluoroscence analysis of HELA cells using Smad2 antibody

Quick Database Links

Gene Symbol

Mothers against decapentaplegic homolog 2

UniProt

Q15796