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SMAD2 Mouse Monoclonal Antibody

SKU: orb738473

Description

The SMAD2 Mouse Monoclonal Antibody is an unconjugated antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, IHC, and WB applications. This antibody targets the Mothers against decapentaplegic homolog 2 in Human samples.

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsFC, IHC, WB
Dilution RangeWestern blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
ReactivityHuman
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.B5E6
ImmunogenE.coli-derived human SMAD2 recombinant protein (Position: E83-Q264).
TargetMothers against decapentaplegic homolog 2
Molecular Weight55 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Concentration500 µg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

hMAD 2; hSMAD2; JV18; JV18 1; MAD homolog 2; Mad related protein 2; MADH2; MADR2; Mothers against DPP homolog 2; SMAD 2; SMAD family member 2; SMAD2

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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