SIX6 Antibody

• Catalog Number: orb214347
• Category: Antibodies
• Description: The SIX6 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SIX6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Gallus, Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SIX6. The exact sequence is proprietary.
• UniProt ID: O95475, Q9QZ28
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: OPTX2; SIX9; Homeobox protein SIX6; Homeodomain pr
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 4990, 20476

Western blot analysis of SIX6 expression in HEK293T (A), HCT116 (B), mouse muscle (C), rat muscle (D) whole cell lysates. (Predicted band size: 35; 27 kD; Observed band size: 27; 43 kD)

Immunofluorescent analysis of SIX6 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.