Anti-SIRPa / CD172a Reference Antibody

• Catalog Number: orb1806109
• Category: Antibodies
• Description: Anti-SIRPa / CD172a Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 145.5 kDa
• Target: SIRPa / CD172a
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-SIRPa / CD172a Reference Antibody (Hospital for Sick Children patent anti-SIRPA) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-SIRPa / CD172a Reference Antibody (Hospital for Sick Children patent anti-SIRPA)is more than 95% ;determined by SEC-HPLC.

Immobilized human SIRPα V1 FC at4 μg/mL can bind Anti-SIRPa / CD172a Reference Antibody (Hospital for Sick Children patent anti-SIRPA);EC50=0.007292μg/mL.

Hospital for Sick Children patent anti-SIRPA FACS Blocking was evaluated using human CCRF-CEM cells. The IC50 was approximately 3.289 ug/ml.nM.