Anti-Shugoshin 1 Antibody

• Catalog Number: orb668090
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Shugoshin 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human Shugoshin 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SGOL1
• Entrez: 151648, 72415
• UniProt ID: Q5FBB7, Q9CXH7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-SGO1 antibody, anti-Shugoshin-like 1 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Shugoshin 1 expression in HT29 (A), mouse testis (B), rat testis (C) whole cell lysates. (Predicted band size: 24; 29; 31; 33; 35; 60; 64 kD; Observed band size: 75 kD)

Immunofluorescent analysis of Shugoshin 1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).