SHIP1 Antibody

SKU: orb44486

Description

Mouse Monoclonal to SHIP-1.

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FC, ICC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: SHIP-01
• Immunogen: Peptide coresponding to a sequence within N-terminal domain of human SHIP-1.
• Target: SHIP1
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IPP5PD, IP5PD, INPP5D

Western blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04; orb43541); Lane 2, 3: immunostaining with anti-human SHIP-1 (SHIP-01); Lane 4, 5: immunostaining with anti-human SHIP-1 (SHIP-02; cat. no. orb44487).

Separation of MOLT-4 cells (red-filled) from CaCo-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cellular suspensions of MOLT-4 and CaCo-2 cell lines stained using anti-SHIP1 (SHIP-01) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Quick Database Links

Gene Symbol

SHIP1

UniProt

Q92835

NCBI Gene ID

3635