SHIP INPP5D Rabbit Monoclonal Antibody

SKU: orb548195

Description

SHIP INPP5D Rabbit Monoclonal Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution range: WB 1:500-2000 IHC 1:50-200 IP 1:20 FC 1:20
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AHG-9
• Immunogen: A synthesized peptide derived from human SHIP
• Target: Cytochrome P450 1A
• Molecular Weight: 87 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1; 3.1.3.86; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP, SHIP1

Immunohistochemical analysis of paraffin-embedded rat spleen, using SHIP Antibody (orb548195) INPP5D was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPP5D Antibody (orb548195) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of SHIP1 expression in Daudi cell lysate (orb548195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D monoclonal antibody (Catalog # orb548195) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for INPP5D.

Quick Database Links

Gene Symbol

Cytochrome P450 1A

UniProt

Q92835