SHARP1 Antibody

SKU: orb224120

Description

The SHARP1 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1

Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD)

Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q99PV5, Q9C0J9, O35779

NCBI Gene ID

79365, 79362

References

Anna Podleśny-Drabiniok # 1, Gloriia Novikova # 1 2, Yiyuan Liu 1, Josefine Dunst 3 4, Rose Temizer 1, Chiara Giannarelli 5 6, Samuele Marro 7 8, Taras Kreslavsky 3 4, Edoardo Marcora 9 10, Alison Mary Goate BHLHE40/41 regulate microglia and peripheral macrophage responses associated with Alzheimer's disease and other disorders of lipid-rich tissues Nat Commun, 15, 2058. (2024)

PubMed: 38448474

Applications

WB

Reactivity

Human