Anti-SHARP1 Antibody

• Catalog Number: orb224120
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SHARP1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BHLHE41
• Entrez: 79362, 79365
• UniProt ID: Q9C0J9, Q99PV5, O35779
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti BHLHB3 antibody, anti DEC2 antibody, anti SHA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Anna Podleśny-Drabiniok # 1, Gloriia Novikova # 1 2, Yiyuan Liu 1, Josefine Dunst 3 4, Rose Temizer 1, Chiara Giannarelli 5 6, Samuele Marro 7 8, Taras Kreslavsky 3 4, Edoardo Marcora 9 10, Alison Mary Goate BHLHE40/41 regulate microglia and peripheral macrophage responses associated with Alzheimer's disease and other disorders of lipid-rich tissues Nat Commun, 15, 2058. (2024)

PubMed: 38448474

Applications

WB

Reactivity

Human

Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD)

Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.