SHANK1a Antibody

SKU: orb1821883

Description

Our SHANK1a rabbit polyclonal primary antibody from Biorbyt is produced in-house. It detects human, mouse, and rat SHANK1a and is antigen affinity purified.

Research Area

Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:500-1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to amino acids from the C-terminal region of human Shank1a protein
• Target: SHANK1a
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 0.02% NaN3
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SH3 and multiple ankyrin repeat domains 1, GKAP/SAPAP interacting protein, Somatostatin receptor interacting protein, SSTR interacting protein, SSTRIP, SPANK1

Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus.

Immunohistochemistry of mouse retinal sections showing specific staining of SHANK1.

Immunohistochemistry of retinal tissue showing specific staining of Shank at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.

Immunohistochemistry of retinal tissue showing specific staining of Shank at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.

Immunohistochemistry showing colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers and the stratum moleculare of the dentate gyrus. Confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 um), the bottom rows (small rectangles) show further enlargements indicated in up.

Immunohistochemistry showing synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence staining of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um.

Western Blot of rat brain lysate showing specific immunolabeling of SHANK1 at 1:500 dilution.

Quick Database Links

Gene Symbol

SHANK1a

UniProt

Q9Y566