Anti-SFMBT1 [RAB-C396]

• Catalog Number: orb1089924
• Category: Antibodies
• Description: Human polyclonal antibody to SFMBT1
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: RAB-C396
• Tested applications: ChIP, ELISA
• Reactivity: Human
• Isotype: Fab fragment
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing SFMBT1 protein under non-denaturing conditions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: SFMBT1
• UniProt ID: Q9UHJ3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: hSFMBT; Scm-like with four MBT domains protein 1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-SFMBT1 antibody RAB-C396. Paraformaldehyde fixed A431 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of RAB-C396 (orb1089947, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of A431 cells with anti-SFMBT1 RAB-C396. Immunofluorescence analysis of paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C396 (orb1089947) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089947, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.