Anti-SERPINA6 [SAIC-42B-10]

• Catalog Number: orb1089975
• Category: Antibodies
• Description: Rabbit monoclonal antibody to SERPINA6
• Target: SERPINA6
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Peptide "AQLLQGLGFNLTER" derived from the Corticosteroid-binding globulin SERPINA6, conjugated to KLH.
• UniProt ID: P08185
• Tested applications: WB
• Clone Number: SAIC-42B-10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Corticosteroid-binding globulin; CBG
• Research Area: Epigenetics
• Note: For research use only

Flow cytometry using the Anti-SERPINA6 antibody SAIC-42B-10. Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-42B-10 (orb1089975, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HepG2 cells with anti-SERPINA6 SAIC-42B-10. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of SAIC-42B-10 (orb1089975) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089975, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.