Anti-SCAP Antibody

• Catalog Number: orb1879479
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SCAP
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SCAP. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/30)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 235623, 22937, 301024
• UniProt ID: Q12770, A2RRU4, Q6GQT6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA0199; Sterol regulatory element-binding protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SCAP expression in Hela (A), HT29 (B), K562 (C), LNCap (D) whole cell lysates. (Predicted band size: 139 kD; Observed band size: 95 kD)

Flow cytometric analysis of K562 cells using Anti-SCAP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.