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Anti-SAF-B1 Antibody

Catalog Number: orb412667

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb412667
CategoryAntibodies
DescriptionRabbit polyclonal antibody to SAFB
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human SAF-B1
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSAFB
Entrez6294, 224903, 64196
UniProt IDD3YXK2, O88453, Q15424
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-HAP antibody, anti-HET antibody, anti-SAFB1 a
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SAF-B1 Antibody

Western blot analysis of SAF-B1 expression in Hela (A), NIH3T3 (B), mouse lung (C), rat brain (D) whole cell lysates. (Predicted band size: 95; 102 kD; Observed band size: 155 kD)

Anti-SAF-B1 Antibody

Immunohistochemical analysis of SAF-B1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SAF-B1 Antibody

Immunofluorescent analysis of SAF-B1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-SAF-B1 Antibody [orb1474358]

    ChIP,  IF,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 30 μl, 200 μl
  • SAFB1 Antibody [orb653414]

    ICC,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • SAFB (C) Antibody, Rabbit Polyclonal [orb131814]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl