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Anti-S6K1 (Phospho-T444) Antibody

Catalog Number: orb224001

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb224001
CategoryAntibodies
DescriptionRabbit polyclonal antibody to S6K1 (Phospho-T444)
TargetRPS6KB1
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Porcine, Rabbit, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T444 of human S6K1 protein. The exact sequence is proprietary.
UniProt IDP67999, Q8BSK8, P23443
Tested applicationsIF, IH, WB
Dilution rangeWB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti STK14A antibody, anti Ribosomal protein S6 ki
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NoteFor research use only
Entrez72508, 6198, 83840
Expiration Date12 months from date of receipt.
Anti-S6K1 (Phospho-T444) Antibody

Western blot analysis of S6K1 (Phospho-T444) expression in HepG2 EGF-treated (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD)

Anti-S6K1 (Phospho-T444) Antibody

Immunohistochemical analysis of S6K1 (Phospho-T444) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-S6K1 (Phospho-T444) Antibody

Immunofluorescent analysis of S6K1 (Phospho-T444) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).