CD8b Antibody (FITC)

SKU: orb154480

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD8B that also known as P37 and LEU2. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Images & Validation

• Tested Applications: FC
• Reactivity: Rat
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 341
• Immunogen: CD8 positive Wistar rat splenic T cell hybridomas
• Target: CD8b
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Ly-3, Ly-C, Lyt-3

Separation of rat CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) rat splenocyte suspension stained using anti-rat CD8b (341) FITC antibody (concentration in sample 1 μg/ml).

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) FITC antibody (concentration in sample 1 μg/ml).

Quick Database Links

Gene Symbol

CD8b

UniProt

P10966

NCBI Gene ID

926